Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
Yoo Chae-Kyung, 전재윤, 김유진, 김성곤, 황경균,
소속 상세정보
( Yoo Chae-Kyung ) - Hanyang University Graduate School of Biomedical Science and Engineering Department of Translational Medicine
전재윤 ( Jeon Jae-Yun ) - Hanyang University College of Medicine Division of Oral and Maxillofacial Surgery
김유진 ( Kim You-Jin ) - Korean Minjok Leadership Academy
김성곤 ( Kim Seong-Gon ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
황경균 ( Hwang Kyung-Gyun ) - Hanyang University College of Medicine Division of Oral and Maxillofacial Surgery
Abstract
Background: The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).
Methods: Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.
Results: Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P??0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P?
Conclusions: These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
키워드
Bone regeneration; Cell adhesion; Cell proliferation; Membrane; Osteoblasts; Silk fibroin
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